Maleimide-(CH2)n-NHS Ester

Maleimide-(CH₂)n-NHS Ester is a heterobifunctional reagent designed for efficient dual conjugation, enabling sequential thiol-specific maleimide coupling and amine-reactive NHS ester binding in a single workflow. Its key advantages include high reactivity for both thiols and amines, tunable spacer length (n) to optimize distance and flexibility, and reduced steric hindrance for enhanced biomolecular assembly, though the NHS ester group requires anhydrous handling due to hydrolysis sensitivity and the maleimide may undergo retro-Michael reactions.